WebbpHIS1525巨大芽孢杆菌表达质粒人源基因质粒 质粒图谱 质粒序列:详询 质粒菌株产品操作说明书 一、扩增流程 收到产品后,请先根据产品管壁标签来判断产品形式,并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。 1、质粒干粉(常温运输,存于-20度,90天保质期,请务必转化挑单克隆培养,不要直接使用和测序) ①收到质粒干粉后请 … http://www.biovector.net/product/1077.html
pHIS1525说明书-分析方法-生物在线 Lab-on-Web
WebbpHIS1525巨大芽孢杆菌表达质粒使用说明: 1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min; 2、从-80℃冰箱中取出相应的感 … Webb冷休克表达载体,pCold I-IV,是利用冷休克基因CSPA启动子设计高效率蛋白质表达载体。. CSPA的启动子的下游处,插入了lac操纵子,以便严格控制表达。. 此外,5'非翻译 … how to select earbuds
巨大芽孢杆菌系统:pHis1525-上海北诺生物科技有限公司
Webb16 mars 2016 · 下游引物获得方法我们先 选择cds区最后20(最好25个)个碱基的序列:gaagggcctgactcagactga。然后把这段序列反向互补得到 新的序 … WebbThe aim of this research was to express the β-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and... DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online … WebbFor further optimization of our project, more vectors are constructed but not listed here. Some things should be mentioned about plasmid pHIS1525, pSPAsp-hp and pHBintE are that, they are all shuttle vectors for E. coli&B. megaterium. And pHBintE is capable of chromosome integration due to its temperature sensitivity. how to select element in iframe with angular