Immortalized human podocyte cell line
Witryna14 lut 2015 · Cell culture and high glucose-induced podocytopathy: An immortalized mouse podocyte cell line 25 which had been generated by expression of a temperature-sensitive mutant of the SV40 Large-T ... WitrynaThe human podocytes derived from the stem cells discussed in this review have shown some advantages over the immortalized podocytes, but their maturation remains a question that has not been compared with that of the human adults in-vivo. 42,43,89 Among the various reports on the induction of podocytes and kidney organoids from …
Immortalized human podocyte cell line
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WitrynaG.J. Audesirk, in Comprehensive Toxicology, 2010 13.24.2.3.5 Immortal cell lines. Immortal cell lines are an alternative to primary cultures. Frequently used cell lines … Witryna17 wrz 2024 · Generally, conditionally immortalized podocytes have lost many of their phenotypes in vivo, including process formation at high cell density with conventional …
Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture. This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. WitrynaThe conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [9,74]. Podocytes were cultured in the medium which contained RPMI 1640 medium with 10% fetal bovine serum, and insulin–transferrin–selenium at 33 °C. For the differentiation, podocytes were …
WitrynaA seminal breakthrough came about a decade ago with the documentation of differentiation in culture of primary rat and human podocytes and the subsequent development of conditionally immortalized differentiated podocyte cell lines that allow deciphering the decisive steps of differentiation and function of ‘in vivo’ podocytes. … WitrynaThe Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex …
Witryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher …
Witryna18 sie 2024 · A conditionally immortalized murine podocyte cell line (E11) was obtained from Cell Line Services. Podocytes were propagated at 33 °C in RPMI1640 supplemented with 10% FBS and 10 U/mL of IFN-γ (Saint Louis, MO, USA) to enhance expression of thermosensitive T antigen. ... Shimada, H.; Rajagopalan, L.E. Rho … green clock wallpaperWitrynaTo investigate changes on the cellular level, we used an immortalized human podocyte cell line and generated a stable knockdown of Sphk2 (Sphk2-kd) by a lentiviral transduction method. green clocks kitchenWitryna11 lis 2009 · Conditionally immortalized human podocytes were cultured for up to 24 h with 375–750 μM palmitate. Functional effects on glucose uptake and ceramide production were measured. ... Reiser J, et al. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc … flow reactionWitrynaLoss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled bran… flow reactflow reactor adalahWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein … flowr delivery hollywood hillsWitryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of … green clockwork headphones