Fitc dmso
WebFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein … WebDespite the growing number of choices in amine-reactive fluorophores, fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) are still widely used reactive fluorescent dyes for preparing fluorescent bioconjugates. ... (DMSO) and should never be used in that solvent. Figure 1.1.8. Reaction of a primary amine with a ...
Fitc dmso
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http://www.delta-f.com/details/1167980 WebAug 1, 2024 · For DMSO/DBTD method, the fluorescent label is tagged to the hydroxyl group of AX. FITC can be grafted onto AX through thiocarbamoyl linkage, as shown in Fig. 1 a. FITC reacts with AX in DMSO at elevated temperature to give O-(fluoresceinyl-thiocarbamoyl) AX with pyridine and DBTD as the catalysts. During the reaction, pyridine …
WebDimethyl sulfoxide, or DMSO, is a highly polar organic solvent that is miscible with water. ... (FITC)) • Thiol-reactive fluorescent labeling reagents (e.g. Alexa Fluor™ 488 or fluorescein maleimide) Molecular Probes™ … WebApr 11, 2024 · 中文名称：荧光素-聚乙二醇-双棕榈酸甘油酯 英文名称：fitc-peg-dpg 外观：取决于分子量 溶解性：water, dmso, dcm 纯度：95% 活性基团：fitc 分子量：400 600 1000 2000 3400 4000 5000 10000 20000 注意事项 取用保持低温干燥，现配现用，避免频繁冻融造成产品失活 存储条件 -20 ...
http://www.protocol-online.org/biology-forums/posts/30123.html WebProduct Details. Preparation. Zombie Green™ Fixable Viability Kit is composed of lyophilized Zombie Green™ dye and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 100 µl of DMSO to one vial of Zombie Green™ dye until fully dissolved. 100 tests = 1 vial of Zombie Green™ + DMSO, 500 tests = 5 vials of Zombie ...
WebFITC is generally supplied as a lyophilized solid and must be resuspended in an organic solvent, such as ethanol, DMSO, or DMF, before labeling proteins or any other amine …
http://vp-sci.com/media/productattachments/files/2/6/267a_pin_tool_volume_fitc_protocol.pdf ear issues with dogsWebDec 11, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 … cssf aifm 2013WebFor example, using 5 mg of IgG and a 10∶1 molar ratio of fluorescein isothiocyanate (FITC) with mol wt of 389: (2) 3. Prepare a 10 mg/mL solution of the fluorophore by weighing 3 or more mg of dye and dissolving it in 0.3 mL or more of DMF or DMSO. Sulfonyl ... DMSO, or ethanol. Dilute this solution approx 1∶1000 in the reaction mixture to ... earist application formWebFluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the … earist application deadlineWebAttach a 20-gauge needle to the tip of a 1 mL syringe. Use this syringe to extract the desired volume of DMSO (use 100 µL per mg of NHS-ester modification solid; depending on the modification, additional DMSO may be necessary). Use the “balloon” technique to maintain the DMSO stock bottle under nitrogen pressure and thereby keep moisture out. earist civil engineeringWebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. cssf aif codeWebFITC, 1g . FITC, 100mg . Chemical name: Fluorescein isothiocyanate: Molecular weight: 389.38 . Extinction coefficient: 70,000M-1: cm-1 ... Dissolve TRITC in DMSO at 1mg/mL. 2. While stirring, slowly add 35µl of TRITC to 1mL of the 6mg/mL antibody solution and mix thoroughly. 2. Incubate for 2 hours at room temperature in the dark. earist cavite courses offered and tuition fee