Web5 Apr 2024 · Therefore, the present study was aimed at hydrolysing stone fish protein with bromelain according to CCD by RSM and study the effect of the process conditions including pH, temperature, E/S ratio and time on DH, DPPH radical-scavenging activity, and Fe 2+ -chelating activity of the resulting hydrolysates. Weband 40°C). The assay is based on the hydrolysis of the polygalacturonic acid substrate measured by titration. Bromelain Units GDU: One gelatin digestion unit per gram of bromelain is that amount of enzyme which liberates 1mg of amino nitrogen from a gelatin substrate in 20 minutes under the conditions of the assay (pH 4.5 and 45°C). The
Optimization of enzymatic treatment of polyamide fabrics by bromelain ...
Web31 Mar 2024 · Response surface methodology (RSM) based on a central composite design was used to model and optimize the degree of hydrolysis (DH) and ACE-inhibitory … Web12 Apr 2024 · The stem region of the plant has broad bromelain (EC 3.4.22.32) specificity for the breakage of proteins whereas fruit bromelain (EC3.4.22.33) has broad specificity for the hydrolysis of protein’s peptide bonds (Campos et al. 2024). The stem bromelain is sequenced and demonstrated to be an individual from the papain superfamily (IUB … harald bruch
Fabrication and characterization of polyvinyl-alcohol-combined ...
WebBromelain is an example of a group of enzymes called proteases. Proteases are protein molecules that can chop up other proteins. (Proteases are very specific in the proteins they chop up, so they don’t chop themselves up!). If bromelain (enzyme) comes in contact with the gelatin (protein) in the hot solution, the bromelain will break down the ... Web7 May 2024 · Hydrolysis was carried out at pH 7 and 50 °C to attain 5, 7, 9 and 11% degree of hydrolysis (DH) and the time was noted. The DH which is defined as the percentage of peptide bonds cleaved by the enzyme was determined according to the method of base consumption using pH stat technique of Adler-Nissen ( 1986 ). Web3 Apr 2012 · The hydrolytic activity of bromelain was evaluated on the basis of the number of carboxylic groups formed on the surface of the polyamide fabrics, and it was measured … harald bottle